Simplified Desalting of Ligation Reactions Immediately Prior to Electroporation into E. coli
نویسندگان
چکیده
منابع مشابه
A simple treatment of DNA in a ligation mixture prior to electroporation improves transformation frequency.
A high frequency of transformation of bacteria of about 1—5xlO cells per /tg of supercoiled plasmid DNA (1, 2) is now routinely obtained when a dense suspension of Escherichia coli (MC1061/P3; Invitrogen) cells ( lxlO 0 cells/ml) are exposed to a brief (3 -5 msec) high voltage electrical pulse. However, if the DNA sample is dissolved in a ligation mixture with a high salt concentration, the tra...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1996
ISSN: 0736-6205,1940-9818
DOI: 10.2144/96216bm12